Composite

Part:BBa_K3747142

Designed by: Anemoon Drinkwaard   Group: iGEM21_Wageningen_UR   (2021-10-21)


nirKV (Nitrite reductase) Pseudomonas stutzeri JM300

Nir is the second enzyme of the denitrification pathway, converting nitrite (NO2-) into nitric oxide (NO). NirK nitrite reductases are copper-dependent. It has long been thought the presence of NirS (a heme-dependent Nir) and NirK was mutually exclusive, however, this is not true as for example Pseudomonas stutzeri possesses both. In contrast to NirS, NirK does not need any other genes for its maturation or for it to be functionally active. Nevertheless, in some organisms, e.g. P. stutzeri JM300, nirK gene is accompanied by nirV gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 534
    Illegal NgoMIV site found at 588
    Illegal NgoMIV site found at 954
    Illegal NgoMIV site found at 1038
    Illegal NgoMIV site found at 1626
    Illegal NgoMIV site found at 1680
    Illegal NgoMIV site found at 2046
    Illegal AgeI site found at 399
    Illegal AgeI site found at 1491
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None